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KMID : 1191520060110010095
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Journal of Korean Traditional Oncology
2006 Volume.11 No. 1 p.95 ~ p.103
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HPLC analysis of Gami-Samhwang-San and prediction of active compounds using QSAR
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Yu Young-Beob
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Abstract
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Objective: Gami-Samhwang-San, a herbal prescription for obesity treatment, is composed of seven crude herbs such as Ephedrae Herba, Scutellariae Radix, Acori Gramineri Rhizoma, Polygalae Radix, Typhae Pollen, Armeniacae Semen, Nelumbo Folium. This study was aimed to evaluate marker substances in n-butanol fraction (SH-21-B) from Gami-Samhwang-San by high performance liquid chromatography (HPLC). And we predicted inhibition activity of major compounds of Gami-Samhwang-San using Quantitative Structure Activity Relationships (QSAR)
Methods: The separation was performed on a YMC J,sphere-H80 CI8(250X4.6 mm I.D) column by gradient elution with H3PO4 buffers in acetonitrile as the moblie phase at a flow-rate of 1.0ml/min.
Results: HPLC was employed to determine the quantities and the qualities of several marker substances such as ephedrine, pseudoephedirne, baicalin, β-αsarone, tenuifoliside, naringenin, amygdalin and hyperoside in the SH-21-B.
Conclusion: We suggest this results could be a useful evidence for quality control of SH-21-B.
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KEYWORD
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HPLC , marker substance , QSAR , Gami-Samhwang-San
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